Can anyone give me some insight into the function of the lac operon in controlling expression of a recombinant protein? I understand how the operon works but don't know how it relates to controlling expression.

2/20/2006 10:36:22 PM

a vector is a circle with arrows

that's as much as i could tell you

sorry

2/20/2006 10:43:13 PM

haha, thanks anyways

2/20/2006 10:44:07 PM

If the operon is on, (because lactose is present in high enough concentrations), the protein is produced. If the protein (actually multiple proteins) are mutated, they wont convert lactose into glucose.

This is assuming the recombinant protein is produced by the lac operon in the first place.

If not, fuck if I know.

2/20/2006 11:24:58 PM

GN 411 test tomorrow?

2/20/2006 11:57:37 PM

nah, just some hw in biotech... figured it out anyway

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2/21/2006 12:31:53 AM